CLONING A SEQUENCE OF SMALL HARPIN RNA DIRECTED TO HUMAN GELATINASE B INTO THE EXPRESSION VECTOR pGPV-17019250

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Название журнала: Восточно Европейский Научный Журнал, Выпуск: , Том: , Страницы в выпуске: -
Автор: Mogulevtseva J.A. Bachelor of Science Russian State Agrarian University – Moscow Timiryazev Agricultural Academy MezentsevA.V. Doctor of Philosophy N.I. Vavilov Russian Institute of General Genetics – IOGen RAS
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Анотация: Summary. INTRODUCTION: Matrix metalloproteinases are a group of zinc-containing calcium-dependent endopeptidases that play a crucial role in the pathogenesis of hyperproliferative disorders, such as psoriasis. In psoriasis, matrix metalloproteinases contribute to epidermal remodeling due to their ability to modify the compo- sition of the extracellular matrix and modulate the intercellular contacts. They also regulate the penetration of dermal microcapillaries by immune cells. AIM of this study was to create a vector that would express small hairpin RNA (shRNA) specific to human gelatinase B and suppress its expression in cultured epidermal keratinocytes. METHODS: shRNA specific to gelatinase B was designed using “RNAi-designer” online tool. The sequence encoding shRNA was cloned into the vector pGPV-17019250 using commercial T4 DNA-ligase and restriction endonucleases BamH1 and EcoRI. The integrity of the obtained expression vector pGPV-17019250-GB was con- firmed by PCR amplification and DNA sequencing with vector-specific primers. RESULTS: In this study, we selected а DNA sequence that encodes shRNA specific to human gelatinase B. We also synthesized and cloned the named sequence into the expression vector pGPV-17019250. In addition, we confirmed that the selected DNA sequence was properly cloned into the vector.
Ключевые слова: Key words: psoriasis, gelatinase B, molecular cloning, expression vector, shRNA, gene silencing.  
Данные для цитирования: Mogulevtseva J.A. Bachelor of Science Russian State Agrarian University – Moscow Timiryazev Agricultural Academy MezentsevA.V. Doctor of Philosophy N.I. Vavilov Russian Institute of General Genetics – IOGen RAS, . CLONING A SEQUENCE OF SMALL HARPIN RNA DIRECTED TO HUMAN GELATINASE B INTO THE EXPRESSION VECTOR pGPV-17019250. Восточно Европейский Научный Журнал. Медицинские науки. ; ():-.



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