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Дата публикации статьи в журнале: 2020/01/08
Название журнала: Восточно Европейский Научный Журнал, Выпуск: 52, Том: 1, Страницы в выпуске: 36-42
Анотация: A key factor in the development of neoangiogenesis in diabetic retinopathy (DR) in patients with type 2 diabetes mellitus (DM2) is Vascular Endothelial Growth Factor (VEGF). The important role of VEGFA gene polymorphisms is highlighted by numerous studies and meta-analyses showing their association with DR, particularly with its proliferative form (PDR), which varies in different populations. Objective. To investigate the relationship between polymorphic genotypes rs2010963 and rs699947 of the VEGFA gene and clinical signs and laboratory parameters of DR in patients with DM2 in the Ukrainian population. The study involved 302 patients with DM2 and DR. The diagnosis was determined according to the International Clinical Classification adopted by the American Academy of Ophthalmology (2003). The control group included 98 people without DM2, DR or other ophthalmic diseases. All patients underwent cataract surgery. The level of VEGFA in intraocular fluid (IOF) collected during the surgery was measured using the enzyme-linked immunosorbent assay (ELISA) method. Analysis of polymorphic DNA loci of the VEGFA gene – rs2010963 and rs699947 – was performed by real-time polymerase chain reaction using unified TaqMan Mutation Detection Assays Thermo Fisher Scientific test systems (USA). Analysis of the results of the study showed that the rs2010963 polymorphism influenced the level of VEGFA in IOF (maximum – in the C/C risk genotype). This polymorphism was associated with gender (C/C genotype was more common in males than females – 3:1), presence of PDR (most comm. only determined in the presence of C/C genotype – 45.4%) and neovascularization of the optic disc (most commonly determined in the presence of G/C heterozygotes – 21.4%). The rs699947 polymorphism had an influence on visual acuity (minimum acuity – in C/C genotype), retinal thickness (maximum value – in C/C genotype), level of VEGFA in IOF (maximum level - in C/C genotype), as well as the presence of PDR and hemophthalmia (most commonly determined in the presence of C/C genotype – 44.7% and 27.7%, respectively). The pathogenic influence of C/C risk genotype of rs2010963 polymorphism was more commonly detected in males, was realized due to the high level of VEGFA in IOF and was manifested by the maximum frequency of PDR. The pathogenic influence of C/C risk genotype of rs699947 polymorphism was also realized due to the high level of VEGFA in IOF, caused decreased visual acuity, retinal thickening and was manifested by the maximum frequency of PDR and vitreous hemorrhage.
Ключевые слова: diabetic retinopathy VEGFA gene polymorphisms rs2010963 rs699947
Данные для цитирования: Zakharevych G.Ye. , . INFLUENCE OF VEGFA GENE POLYMORPHISMS RS2010963 AND RS699947 ON CLINICAL AND LABORATORY INDICATORS IN DIABETIC RETINOPATHY AMONG PATIENTS WITH TYPE 2 DIABETES MELLITUS (36-42). Восточно Европейский Научный Журнал. Медицинские науки. 2020/01/08; 52(1):36-42.